Recombinant stable MM.1S cell line constitutively expressing a firefly luciferase and enhanced GFP (eGFP) cassette driven by a CMV promoter.
Mycoplasma testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Host Species: human
Supplied as Each vial contains 2 x 106 cells in 1 ml of cell freezing medium (Biohippo, #79796)
Background
MM.1S are multiple myeloma human B lymphoblasts. The parent cell line MM.1 was established from the peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy. MM.1S cells are sensitive to dexamethasone.The cells were infected with an integrating Firefly luciferase-eGFP lentivirus (Biohippo #79980-G) and cloned by limited dilution to get a monoclonal population. The cells stably express both the Firefly luciferase and enhanced GFP genes, making these cells an excellent tool to measure specific killing by CAR-T or NK cells.
Application
Use as a control for killing assays Suitable as a B-cell target for CAR-T or CAR-NK cells for optimizing CAR design
Host Cell
MM.1S
Shipping Temperature
-80°C (dry ice)
Note
License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit Biohippobioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected] Warning: Avoid freeze/thaw cycles.
Storage Stability
Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.