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Home / Cells / Cell Lines

Firefly Luciferase Raji Cell Line

SKU : BHC18200166

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BPS Bioscience

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Mfr.No.	78622
Description	Recombinant Raji cells constitutively expressing firefly (Photinus pyralis) luciferase under the control of a CMV promoter. Mycoplasma testing: The cell line has been screened to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains 2 x 10⁶ cells in 1 ml of 10% DMSO
Background	The Raji line was established from a Burkitt's lymphoma patient. Raji cells constitutively express B cell antigens CD19, CD20, and CD22, and offer a physiologically relevant platform to evaluate cancer-directed immunotherapies such as Chimeric Antigen Receptor (CAR) T-cells. The Firefly Luciferase - Raji Recombinant Cell Line makes an excellent target for CAR-T or NK cells targeting CD19, CD20, or CD22. The signal generated by the firefly luciferase reporter is proportional to Raji cell numbers and facilitates the quantification of Raji cells killing upon co-culture with CAR-T or NK cells.
Application	1. Use as an internal control in CAR-T or NK co-culture killing assays 2. In vitro and in vivo Bioluminescence Imaging
Formulation	Media Required for Cell Culture Thaw Medium 2 (Biohippo #60184): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.Growth Medium 2D (Biohippo #79639): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 200 ug/ml of Hygromycin B.
Host Cell	Raji, a human B lymphoblastoid cell line derived from a patient with Burkitt's lymphoma, suspension
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit Biohippobioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]. Warning: Avoid freeze/thaw cycles.

Storage Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200166	78622	2 vials	$2249.50		Add to Cart

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