Sox17 is a transcription factor that regulates endodermal differentiation of stem cells in the developing human embryo. The endoderm is one of the primary germ layers that appears early in embryonic development. The endoderm gives rise to the lining of the digestive tract, respiratory tract and endocrine organs. Human Sox17 Expressing Embryonic Stem Cells are endoderm progenitors and exhibit markers of the definitive endoderm. Sox17 plays a critical role in general organogenesis of the endoderm during embryonic development and this cell line remains able to undergo hepatic and pancreatic differentiation.
Cell Type
Drug Discovery Cell Lines
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1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Use of PriCoatTM T25 Flasks (G299) or Applied CellExtracellular Matrix (G422) is required for cell adhesion to theculture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the PropagationRequirements below.The base medium for this cell line is Prigrow III medium availableat?abm, Cat. No.?TM003. To make the complete growth medium, addthe following components to the base medium: KnockOut? Serum Replacement (Gibco) to a final concentration of 15%and Penicillin/Streptomycin Solution (G255) to a final concentration of1%.Change media every 2-3 days.Carbon dioxide(CO2): 5%, Temperature: 37.0°C.
Reviews of Conditionally Immortalized Stria Vascularis Cell Line (SV-k1)