Recombinant CD5-CHO K1 cell line stably expressing a full-length human CD5 receptor (accession number: NM_014207.3). Surface expression of hCD5 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of hCD5 [removed]High, Medium and Low) to mimic different stages of cancer target cells with various CD5 expression levels.
Mycoplasma testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Host Species: hamster
Supplied as Each vial contains ~2 x 106 cells in 1 ml of 90% FBS, 10% DMSO
Background
CD5 is a type-1 transmembrane glycoprotein with an extracellular region composed of three scavenger receptor, cysteine-rich domains. This lymphocytic-specific scavenger receptor functions at the interphase of the innate and adaptive immune responses. It is involved in: (i) microbial-associated pattern recognition; and (ii) modulation of intracellular signals mediated by antigen-specific receptor present in T and B cells. CD5 is expressed by all T cells and some B cell subsets (B1a and Breg or B10). T cells usually express higher levels of CD5 than B cells, and CD5 is upregulated in T cells upon strong activation. CD5 is a signaling co-receptor, associated with the antigen-specific receptor complex of T and B cells, and down-modulates the activation/differentiation signals delivered upon specific antigen recognition. The signaling pathway used by CD5 is only partially known.
Application
Screen for activators or inhibitors of antibody-mediated signaling for immunotherapy research and drug discovery. Characterize CD5 antibodies and ligands.
Formulation
Thaw Medium 3 (Biohippo #60186): F-12K medium (Hyclone, #SH30526.01) supplemented with 10% FBS, 1% Penicillin/Streptomycin (Hyclone, #SV30010.01)Growth Medium 3B (Biohippo #79529): Thaw Medium 3 (Biohippo, #60186) plus 500ug/ml of Hygromycin B.
License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit Biohippobioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]. Warning: Avoid freeze/thaw cycles.
Storage Stability
Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.