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CD27 CHO-K1 Stable Recombinant Cell Line

SKU : BHC18200049

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BPS Bioscience

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Mfr.No.	60624
Description	CHO-K1 cell line expressing full length human CD27 (TNFRSF7). Expression is confirmed by real time qPCR and Western blot.Screening for antibodies recognizing CD27 and screening antibodies for binding affinities. Since CHO-K1 cells do not express endogenous CD27, this cell line serves as an excellent model for studying changes in CD27-mediated signaling pathways, such as NF-B, MAPK and SAPK/JNK. Mycoplasma testing: This cell line has been screened using the MycoAlert Mycoplasma Detection Kit (Lonza, Cat.#LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set(Lonza, Cat. #LT07-518) was used as a positive control. Host Species: hamster Supplied as Each vial contains ~2 X 10^{6 cells in 1 ml of 10% DMSO.}
Background	CD27 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes theT cell co-stimulatory receptors OX40, 4-1BB and herpesvirus entry mediator (HVEM). CD27 isexpressed on various types of T cells, B cells and a subset of natural killer cells. It activates NF-B and MAPK/JNK signaling upon interaction with its TNF-like ligand, CD70, which is expressedby numerous tumor cells. Adaptor proteins TRAF2 and TRAF5 can also stimulate CD27signaling. Activation of CD27 leads to lymphoid proliferation, differentiation, apoptosis, and theinduction of long-term memory. The CD27/CD70 pathway is a key target for the development oftreatments for cancer and inflammatory diseases.
Host Cell	CHO-K1
Synonyms	CD70 antigen, Tumor Necrosis Factor Ligand Superfamily Member 7, TNFSF7, CD27 Ligand, Ki-24 antigen, CD27-L, CD27LG,
Shipping Temperature	-80°C (dry ice)
Note	Culture Medium F-12KK (Thermo Fisher, at. #21127022) 10% heat-inactivated FBS, 1x Penicillin/ Streptomycin (Thermo Fisher, at. #10378016), 1 mg/ml Geneticin (G418) License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc.

Product Format	Aqueous solution containing DMSO
Storage Stability	Store in liquid nitrogen immediately upon receipt.

Reference	1. Akiba H et.al. (1998) CD27, a member of the tumor necrosis factor receptor superfamily, activates NF-B and stress-activated protein kinase/ c-Jun N-terminal kinase via TRAF2, TRAF5, and NF-B inducing kinase. J. Biol. Chem. 273: 13353 2. Yamamoto H et.al. (1998) NF-B activation in CD27 signaling: Involvement of TNF receptor-associated factor in its signaling and identification of functional region of CD27. J. Immunology 161: 4753-4759

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200049	60624	2 vials	$4950		Add to Cart

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