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CD1 Mouse Primary Hepatocytes - Plateable

SKU : BHC16400481

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Cell Biologics, Inc.

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Mfr.No.	CD-1224F
Description	CD1 Mouse Primary Hepatocytes from Cell Biologics are isolated from liver tissues of pathogen-free laboratory CD1 mice. Cells at passage 0 are cryo-preserved and each vial contains 3x10^6 cells per ml. CD1 Mouse Primary Hepatocytes are characterized by immunofluorescence staining with an antibody of ZO-1. Cells can be plated on culture plates for desired experiments under the cell culture conditions specified by Cell Biologics. Primary hepatocytes can not be cultured indefinitely and can not be passaged.
Cell Origin	Mouse
Cell Type	Hepatocyte
Freeze Thaw Recovery	Thaw the cells quickly (<2 min) in a 37°C water bath until just prior to complete thawing.Wipe the outside of the vial with 70% ethanol.Gently resuspend the cells and transfer cells (3 million cells) to a 15 ml sterile tube.Add 7 ml of pre-warmed Cell Biologics Cell Culture Medium (Catalog number M1265, Cell Biologics) gently to the side of the tube and slowly pipette up and down 2 to 3 times to resuspend the cells.Carefully pour cell suspension into 2 wells of 6-well-plate (0.7-1.5 million cells per well of 6-well plate).Once the cells have attached to the culture dishes about 70-80% (after 2-8h), gently aspirate supernatant (remove cell debris and non-adherent cells) and replace the pre-warmed Maintenance Medium (Catalog number M1265, Cell Biologics).Cells should be checked daily under a microscope to verify appropriate cell morphology. Proceed with experiment assays within 1-3 days (when cells have reached 90-100% confluence) after plating cells.Note: The numbers of seeding cells in each well may need to be modified according to the user's experience. Recommended seeding density: 200,000 viable cells/cm^2.
Media	Complete Hepatocyte Medium /w Kit (500 ml)
Tissue	Liver
Notes	Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Species	Mouse
Species details	CD1 Mouse

Applications	Hepatologic Research

Product Use	CD1 Mouse Primary Hepatocytes from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-150°C) immediately until ready for use. Cells should be plated and used directly for desired studies within 1-2 weeks upon receipt. Primary cells can never be kept at -20 °C or -80 °C freezer.

Product Manual	Cell Culture Protocol MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400481	CD-1224F	3x106 Cells	$560		Add to Cart

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