JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Cells / Cell Lines

Cas9-Expressing MDA-MB-231 Cell Line (Low Expression)

SKU : BHC18200251

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

BPS Bioscience

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	78150-L
Description	This cell line is a clonal derivative from the Cas9-Expressing MDA-MB-231 Cell Pool (Biohippo, #78069). It was generated by limited limiting dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a low- or high-expressing cell line. The expressed Cas9 protein includes a C-terminal FLAG tag. Mycoplasma testing: This cell pool has been screened using the MycoAlert Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control. Host Species: human Supplied as Each vial contains ~2 x 10^6 cells in 1 ml of FBS with 10% DMSO.
Background	Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing MDA-MB-231 cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.
Application	1. Quickly generating knock-out cell pools or cell lines in MDA-MB-231 cell pools. 2. Implementing sgRNA screens in Cas9 expressing MDA-MB-231 cell pools.
Host Cell	MDA-MB-231
Species	S. pyogenes
Synonyms	SpCas9 cell line, CRISPR cell line, Cas9 cell line, MDA-MB-231 cells, Human metastatic mammary adenocarcinoma cells, 78150-L, 78150-H
Shipping Temperature	-80°C (dry ice)
Note	License DisclosureVisit Biohippobioscience.com/license for the label license and other key information about this product.Troubleshooting Guide: For all questions, please email [email protected] CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights.

Storage Stability	Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Product Manual	Datasheet

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHC18200251	78150-L	2 vials	$7150		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.