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Cas9-Expressing Human Primary Dermal Microvascular Endothelial Cells

SKU : BHC16401972

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Cell Biologics, Inc.

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Mfr.No.	H-6064Cas9
Description	Cas9-Expressing Human Primary Dermal Microvascular Endothelial Cells from Cell Biologics are isolated from human skin tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10^6 cells per ml and is delivered frozen. Cas9-Expressing Human Primary Dermal Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31/PECAM-1 (Catalog No. FAB3567P, R&D), or VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Cell Type	Endothelial Cells
Cell Models	Cas9
Media	Complete Human Endothelial Cell Medium w/ Kit 500 ml
Tissue	Dermal Microvascular
Notes	Investigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at
Species	Human

Applications	Dermatologic Research

Product Use	Cas9-Expressing Human Primary Dermal Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Product Manual	Cell Culture Protocol MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHC16401972	H-6064Cas9	0.5x106 Cells	$960		Add to Cart

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