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Home / Cells / Primary Cells

Cas9-Expressing Human Primary Colonic Fibroblasts

SKU : BHC16402012

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Cell Biologics, Inc.

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Mfr.No.	H-6231Cas9
Description	Cas9-Expressing Human Primary Colonic Fibroblasts from Cell Biologics are isolated from human colonic tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10^6 cells per ml and is delivered frozen. Cas9-Expressing Human Primary Colonic Fibroblasts are characterized by their spindle morphology and immunofluorescent staining with anti-fibroblast-specific protein 1 or anti-Vimentin antibody. These cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Cell Type	Fibroblasts
Cell Models	Cas9
Media	Complete Fibroblast Medium w/ Kit 500 ml
Tissue	Colonic
Notes	Investigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
Species	Human

Applications	Digestive Research

Product Use	Cas9-Expressing Human Primary Colonic Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Product Manual	Cell Culture Protocol MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHC16402012	H-6231Cas9	0.5x106 Cells	$960		Add to Cart

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