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Cas9-Expressing HCT116 cell pool

SKU : BHC18200076

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BPS Bioscience

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Mfr.No.	78073
Description	Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing HCT116 cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines. Mycoplasma testing: This cell line has been screened using the MycoAlert Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control. Host Species: human Supplied as Each vial contains ~2 x 10^6 cells in 1 ml of FBS with 10% DMSO.
Application	1. Quickly generating knock-out cell pools or cell lines in HCT116 cells. 2. Implementing sgRNA screens in Cas9-expressing HCT116 cells.
Host Cell	HCT116
Species	S. pyogenes
Synonyms	SpCas9 cell pool, CRISPR cell pool, Cas9 cell pool, HCT116 cells, Human Colorectal Carcinoma Cells
Shipping Temperature	-80°C (dry ice)
Note	The CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights.

Storage Stability	Immediately upon receipt, store in liquid nitrogen.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200076	78073	2 million cells	$874.50		Add to Cart

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