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Cas9-Expressing HCT116 Cell line High expression

SKU : BHC18200242

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BPS Bioscience

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Mfr.No.	78135-H
Description	This cell line is a clonal derivative from the Cas9-Expressing HCT116 Cell Pool (Biohippo, #78073). It was generated by limited dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a high-expressing cell line. The expressed Cas9 protein includes a C-terminal FLAG tag. Mycoplasma testing: This cell line has been screened using the MycoAlert Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control. Host Species: human Supplied as Each vial contains ~2 x 10^6 cells in 1 ml of FBS with 10% DMSO.
Background	Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing HCT116 cell lines can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.
Application	1. Quickly generating knock-out cell pools or cell lines in HCT116 cells. 2. Implementing sgRNA screens in Cas9-expressing HCT116 cells.
Host Cell	HCT116
Tag	C-terminal FLAG-tag
Uniprot	Q99ZW2
Species	S. pyogenes
Synonyms	SpCas9 cell line, CRISPR cell line, Cas9 cell line, HCT-116 human colorectal carcinoma cell line
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product.Troubleshooting Guide: For all questions, please email [email protected] CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights. Warning: Avoid freeze/thaw cycles.

Storage Stability	Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200242	78135-H	2 vials	$7150		Add to Cart

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