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Cas9-Expressing Daudi Cell Line

SKU : BHC18200088

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BPS Bioscience

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Mfr.No.	78157
Description	This cell line is a clonal derivative from the Cas9-Expressing Daudi Cell Pool (Biohippo, #78089). It was generated by limiting dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a high-expressing cell line. The expressed Cas9 protein includes a C-terminal FLAG tag. Mycoplasma testing: This cell line has been screened using the MycoAlert Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control. Host Species: human Supplied as Each vial contains ~2 x 10^6 cells in 1 ml of FBS with 10% DMSO.
Background	Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing Daudi cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.
Application	1. Quickly generating knock-out cell pools or cell lines in Daudi cells. 2. Implementing sgRNA screens in Cas9 expressing Daudi cells.
Host Cell	Daudi
Species	S. pyogenes
Synonyms	SpCas9 cell line, CRISPR cell line, Cas9 cell line, Burkitt's lymphoma B-cells, B-cell non-Hodgkin lymphoma, NHL
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure : Visit Biohippobioscience.com/license for the label license and other key information about this product.Troubleshooting Guide: For all questions, please email [email protected] CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights.

Storage Stability	Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200088	78157	2 vials	$7150		Add to Cart

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