C57BL/6 Mouse Primary Embryonic Fibroblasts can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer
Although C57BL/6 Mouse Embryonic Fibroblasts are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Frozen
1×106cells
General Information
C57BL/6 Mouse Primary Embryonic Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 (Millipore USA) by immunofluorescence staining. C57BL/6 Mouse Primary Embryonic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.