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C57BL/6 Mouse Primary Coronary Artery Smooth Muscle Cells

SKU : BHC16400623

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Cell Biologics, Inc.

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Mfr.No.	C57-6225
Description	C57BL/6 Mouse Primary Coronary Artery Smooth Muscle Cells from Cell Biologics are isolated from the coronary artery tissues of pathogen-free laboratory C57BL/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10^6 cells per ml and is delivered frozen. C57BL/6 Mouse Primary Coronary Artery Smooth Muscle Cells are characterized by immunofluorescent staining with antibody of ?-smooth muscle actin (A2547, Sigma). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Cell Origin	Mouse
Cell Type	Smooth Muscle Cells
Application	C57BL/6 Mouse Primary Coronary Artery Smooth Muscle Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Although C57BL/6 Mouse Primary Coronary Artery Smooth Muscle Cells are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Frozen	1×10⁶cells
General Information	C57BL/6 Mouse Primary Coronary Artery Smooth Muscle Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Media	Complete Smooth Muscle Cell Medium w/ Kit 500 ml
Tissue	Coronary Artery
Notes	Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Species	Mouse

Applications	Cardiovascular Research

Product Use	C57BL/6 Mouse Primary Coronary Artery Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Product Manual	Cell Culture Protocol MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400623	C57-6225	0.5x106 Cells	$510		Add to Cart

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