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C57BL/6 Mouse Primary Aortic Endothelial Cells

SKU : BHC16400301

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Cell Biologics, Inc.

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Mfr.No.	C57-6052
Cell Origin	Mouse
Cell Type	Endothelial Cells
Application	C57BL/6 Mouse Primary Aortic Endothelial Cells can be used in assays of cell-cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Although C57BL/6 Mouse Primary Aortic Endothelial Cells are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Frozen	1×10⁶cells
General Information	C57BL/6 Mouse Primary Aortic Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R&D System) or CD31/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. C57BL/6 Mouse Primary Aortic Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Tissue	Aortic
Species	Mouse
Species details	C57BL/6 Mouse

Applications	Cardiovascular Research

Product Manual	Cell Culture Protocol MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400301	C57-6052	1x106 Cells	$590		Add to Cart

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