c-Kit or stem cell factor (SCF) receptor is a type III tyrosine kinase receptor that has a major role in the hematopoietic system. The c-Kit wild-type-expressing Ba/F3 cells (with GNNK- isoform) are ideal in studies in relation to c-Kit function, mechanisms, and biological activities that may lead to diseases. The GNNK- isoform is the outcome of the c-Kit gene alternative splicing sites, resulting in different isoforms of the tetrapeptide GNNK, and is the main transcript found in human malignancies. Signaling molecules Cbl, Shc, Gab2, Erk and Akt are more strongly activated by the presence of the GNNK- isoform. These cells will proliferate in the presence of the ligand SCF and activate downstream signal transduction. Rapid degradation of the SCF is observed in cells expressing the GNNK-, as the Src inhibitor SU6656 (SUGEN) exerts stronger effects when compared with GNNK isoform cells. Apart from the GNNK- isoform, GNNK isoform c-Kit wild-type Stable Ba/F3 cells are also available (Cat. No. T3042). Together with c-Kit mutated (i.e. D816V, Y568F, Y570F, Y703F) GNNK- isoform Ba/F3 cells, these cell lines are suitable for studies in drug discovery for mutations in the c-Kit receptor which are associated to oncogenic and other diseases.
Cell Type
Drug Discovery Cells
Disclaimer
1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Growth Properties
Suspension
Quantity
1x106 cells / 1.0 ml
Tissue
Bone Marrow
Morphology
Round
Population Doubling
15 - 25 hours
Species
Mouse (M. musculus)
Propagation
The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the completed growth medium, add thefollowing components to the base medium: 10 ng/ml recombinant murine IL-3, fetal bovine serum (TM999) to a finalconcentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of1%. Puromycin can be added to a final concentration of 1.2 mug/mL, but is not required for propagation.Carbon dioxide(CO2): 5%, Temperature: 37.0°C.
Quality Control
1) Immunoprecipitation and western blotting for presence of c-Kit and isoform GNNK-; 2) Cell survival assay with stem cell factor (SCF)
Reviews of c-Kit/WT Stable Ba/F3 (with GNNK+ isoform) Cell Line