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B129 Mouse Primary Spleen Fibroblasts

SKU : BHC16400614

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Cell Biologics, Inc.

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Mfr.No.	B129-7022
Description	B129 Mouse Primary Spleen Fibroblasts from Cell Biologics are isolated from spleen tissue of pathogen-free laboratory B129S2/SvPasCrl mice. B129 Mouse Primary Spleen Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded.Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10⁶ cells per ml and is delivered frozen.
Cell Origin	Mouse
Cell Type	Fibroblasts
Application	B129 Mouse Primary Spleen Fibroblasts can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Although B129 Mouse Spleen Fibroblasts are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Frozen	1×10⁶cells
General Information	B129 Mouse Primary Spleen Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 (Millipore USA) by immunofluorescence staining. B129 Mouse Primary Spleen Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Tissue	Spleen

Product Use	B129 Mouse Primary Spleen Fibroblasts fromCell Biologics are distributed for research purposes only. Our products are not authorized for human use, forin vitrodiagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request. Never can primary cells be kept at -20 °C.

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400614	B129-7022	Frozen Vial	$510		Add to Cart

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