B129 Mouse Bone Marrow Mesenchymal Stem Cells from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory B129 mice and grown in tissue culture flasks with Cell Biologics Complete Growth Medium. Cells at passage 1 are harvested from flasks and cryo-preserved in vials. Each vial contains 1x10^6 cells per ml. B129 Mouse Bone Marrow Mesenchymal Stem Cells are characterized by immunofluorescence staining with antibody of CD44, Sca-1 or CD29. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Cell Origin
Mouse
Cell Type
Mesenchymal Stem Cells
Application
Mouse Bone Marrow Mesenchymal Stem Cells can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, or cell derivatives for desired research applications.
Frozen
0.5×106cells
Media
M5556 Mesenchymal Cell Medium w/ Kit (500 ml)
Tissue
Bone Marrow
Notes
Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Species
Mouse
Species details
B129 Mouse
Applications
Hematopoietic Research, Immunologic Research
Product Use
B129 Mouse Bone Marrow Mesenchymal Stem Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage
Cryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.