B129 Mouse Bone Marrow Dendritic Cells are derived from the tibias and femurs of pathogen-free laboratory adult mice. Cells are grown in 100 mm treated tissue culture dish and incubated in Cell Biologics Culture Complete Growth Medium for 5-7 days. Prior to shipping, cells are detached from dishes and immediately cryo-preserved in vials. Each vial contains at least 5 x106 cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of markers using antibodies, CD11c by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Cell Biologics.
Cell Origin
Mouse
Cell Type
Dendritic Cells
Application
Mouse Bone Marrow Dendritic Cells can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications.
Disclaimer
Investigators should handle the Cells that you receive from Cell Biologics with caution and treat all Cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Frozen
5×106cells
Tissue
Bone
Product Use
Mouse Bone Marrow Dendritic Cells from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics' Cells or Products from the purchaser to other markets, organizations, or individuals is prohibited by Cell Biologics. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage
Cell Biologics will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.