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ARE Reporter HepG2 Cell line (Nrf2 Antioxidant Pathway)

SKU : BHC18200018

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BPS Bioscience

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Mfr.No.	60513
Description	The ARE Reporter - Hep G2 cell line is designed to monitor Nrf2 antioxidant response pathway. The cell contains a firefly luciferase gene under the control of ARE (antioxidant response element) stably integrated into Hep G2 cells. Mycoplasma testing: The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains ~2 X 10^6 cells in 1 ml of 10% DMSO.
Background	The Nrf2 antioxidant response pathway plays an important role in cellular antioxidant defense. Nrf2, a basic leucine zipper transcription factor, induces the expression of antioxidant and phase II enzymes by binding to the ARE (antioxidant response element) region of the gene promoter. Under basal conditions, Nrf2 is retained in the cytosol by binding to the cytoskeletal protein Keap1. Upon exposure to oxidative stress or otherARE activators, Nrf2 is released from Keap1 and translocates to the nucleus, where it can bind to the ARE, leading to the expression of antioxidant and phase II enzymes that protect the cell from oxidative damage.
Application	Monitor Nrf2 antioxidant response pathway activity. Screen for activators or inhibitors of the Nrf2 antioxidant response pathway.
Biological Activity	This cell line is validated for the response to the stimulation of tert-butylhydroquinone and sulforaphane.
Host Cell	HepG2
Uniprot	Q16236
Synonyms	ARE cell line, Nrf2 reporter, Nrf
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Product Format	aqueous solution containing DMSO
Storage Stability	Store in liquid nitrogen immediately upon receipt.

Reference	1. Lee, JM et al. (2004) An important role of Nrf2-ARE pathway in the cellular defense mechanism. J Biochem Mol Biol. 37(2): 139-143. 2. Dinkova-Kostova, AT et al. (2002) Direct evidence that sulfhydryl groups of Keap1 are the sensors regulating induction of phase 2 enzymes that protectagainst carcinogens and oxidants. Proc Natl Acad Sci U S A. 99(18): 11908-11913.

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200018	60513	2 vials	$2689.50		Add to Cart

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