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Home / Cells / Primary Cells

Aged Rat Proximal Tubular Epithelial Cells

SKU : BHC16401264

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Cell Biologics, Inc.

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Mfr.No.	AR-6015
Description	Aged Rat Primary Proximal Tubular Epithelial Cells from Cell Biologics are isolated from proximal tubular tissue of 2-year old laboratory SpragueDawley rat. Aged Rat Primary Proximal Tubular Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 0.5x10⁶ cells and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.
Cell Origin	Rat
Cell Type	Epithelial Cells
Application	Standard biochemical procedures performed with epithelial cell cultures include the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Frozen	0.5×10⁶cells
General Information	Aged Rat Primary Proximal Tubular Epithelial Cells are characterized by immunofluorescent staining with antibody of E-cadherin (Anti-E-Cadherin Antibody, sc-8426, Santa Cruz) or ZO-1 (Anti-ZO-1 Antibody, MABT11, clone R40.76, Millipore). Aged Rat Primary Proximal Tubular Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Tissue	Proximal Tubular

Product Use	Aged Rat Primary Proximal Tubular Epithelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells can be never kept at -20 °C.

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SKU	Supplier No.	Size	Your price	Quantity
BHC16401264	AR-6015	Frozen Vial	$650		Add to Cart

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