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TMEM16A-HEK293 Cell Line

SKU : BHC18200231

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Mfr.No.	90230
Description	Stable, recombinant HEK293 cell line expressing human TMEM16A (transmembrane protein 16A, also called anoctamin 1, calcium-activated chloride channel (ANO1), accession number NM_018043). Mycoplasma testing: The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains ~1 X 10^6 cells in 1 ml of 10% DMSO.
Background	Calcium-activated chloride channels (CaCCs) are major regulators in numerous physiological processes including sensory transduction, epithelial secrection, cardiac and neutonal excitation, and smooth muscle contraction. TMEM16A (ANO1), a member of a family of putative plasma membrane proteins, is identified as a CaCC that is activated by intracellular Ca²⁺ and Ca²⁺-mobilizing stimuli. It has eight putative transmembrane segments without domains evidently involved in calcium regulation. The relative permeability of TMEM16A to monovalent anions is NO₃> I>Br> Cl> F.
Application	Drug compound screening for activators or inhibitors of TMEM16A Functional assays for TMEM16A Efficient antigen for mouse immunization
Biological Activity	N-terminal FLAG tagged human TMEM16A channel has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The CaCC activity of TMEM16A was characterized by an assay based on halide-sensitive yellow fluorescent protein (YFP) mutant whose fluorescence is quenched by increasing halide concentration. When TMEM16A-expressed HEK293 cells were stimulated with ionomycin to raise the intracellular level of Ca²⁺, TMEM16A produced I^- influx in HEK293 that triggered the rapid decreaseof fluorescence of transfected YFP mutant. The ionomycin-induced I^- influx through TMEM16A was blocked by niflumic acid, a CaCC channel blocker.
Host Cell	HEK293
Uniprot	Q5XXA6
Species	Human
Synonyms	TMEM16A, transmembrane protein 16A, anoctamin 1, calcium-activated chloride channel, ANO1
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Storage Stability	Store in liquid nitrogen immediately upon receipt.

Reference	1. Verkman A.S. and Galietta L.J.V. (2009) Cholide channels as drug targets. Nature Reviews 8: 153-171. 2. Caputo A. et al. (2008). Science 322: 590-594. 3. Yang Y.D. et al. (2008). Nature 455: 1210-1215.

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200231	90230	2 vials	$10340		Add to Cart

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