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TCF/LEF StemBright Luciferase iPS Cell Pool (Wnt Pathway)

SKU : BHC18200144

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BPS Bioscience

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Mfr.No.	78515
Description	The TCF/LEF (T-cell factor/lymphoid enhancing factor) StemBright Luciferase Cells contain a firefly luciferase gene under the control of TCF/LEF responsive elements, stably integrated into induced Pluripotent Stem (iPS) cells. TCF/LEF transcription factors are downstream of the Wnt signaling pathway. This cell pool is validated for its response to GSK3beta inhibitor CHIR-99021, which activates the Wnt signaling pathway in human pluripotent stem cells. Mycoplasma testing: The cell line has been screened to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains 2 x 10⁶ cells in 1 ml of iPS cell freezing medium
Background	The Wnt signaling pathway is a well described regulator of organism development and cell fate specification. Cellular pluripotency and differentiation of multiple lineages, including cardiomyocytes and neurons are modulated by this pathway. There are 19 Wnt ligands and 10 Frizzled receptors, which stimulate canonical as well as non-canonical Wnt pathways. In the canonical pathway, cell activation by a Wnt ligand leads to the stabilization and nuclear translocation of beta-catenin and subsequent activation of TCF/LEF transcription factor. The Wnt signaling pathway has recently been shown to play a role in cell cycle reentry and proliferation of iPS-derived cardiomyocytes. It is a therapeutic target in the treatment of neuropsychiatric disorders.
Application	Screen for Wnt-activating small molecules in iPS cells
Host Cell	Non-Disease Human iPS Cell Line (iXCells 30HU-002)
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: The iPSC technology is protected by several patents, including US patent Nos. 8048999, 8058065, 8129187, 8278104, 8530238, 8900871, 9404124, 9499797, 10519425, and patent pending, for which iPS Academia Japan, Inc. has been granted license rights with a sub-licensable right.The purchase of this cell line grants you a 10-year license to use this cell line in your immediate laboratory for internal research purposes. Commercial use of this cell line is not allowed. Commercial use requires the appropriate license from iPS Academia Japan, Inc.This cell line is for research use only, not for therapeutic or prophylactic use in humans or animals. Use in humans is strictly prohibited.This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of the cell line in any way. Modification of this cell line or transfer to another facility of the cells requires a separate license or additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc.Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use and does not accept any liability in connection with the handling or use of the cell line.Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide : Visit Biohippobioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Storage Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. As this is a cell pool and not a cell line, Biohippo cannot guarantee the stability of the genetic modifications over time. Clonal derivation may occur. We recommend freezing cell vials very early on and growing the cells for a limited number of passages. Cells should be cultured using Growth Media, which contain selection antibiotics.

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200144	78515	2 vials	$9350		Add to Cart

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