Recombinant HEK293 cell transfected with tetracycline repressor gene. This cell line is validated for response to the stimulation of doxycycline.
Host Species: human
Background
The T-REx cell line expresses the tetracycline repressor protein. Cells are transfected with the gene of interest under control of a minimal promoter and the tet operon. The Tet repressor protein binds to the operon and represses transcription. Tetracycline regulation is based on the binding of either tetracycline (or doxycycline) to the Tet repressor protein, which de-represses the promoter controlling expression of the gene of interest.
Host Cell
HEK293
Species
Human
Shipping Temperature
-80°C (dry ice)
Note
Just after thawing and at low density, the cells may grow at a slower rate. It is recommended to split the cells with ~ 1:4 ratio at the beginning of culturing. After several passages, the cell growth rate increases and the cells can be split with 1:8 -1:20 ratio weekly.License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.
Product Format
Each vial contains 1.5 X 10^6 cells in 1 ml of 10% DMSO
Storage Stability
Store in liquid nitrogen immediately upon receipt.
Reference
On the use of the T-REx tetracycline-inducible gene expression system in vivo. Dobrovolsky VN1, Heflich RH. 2007. Biotechnol Bioeng. 98(3):719-23.