Tumorigenecity: yes, in newborn rats and chickensIsoenzyme: Histopathology: sarcomaSubculture: Remove medium, add fresh 0.25 % Trypsin for 1 to 3 minutes, remove medium allow the culture to sit at room temp. For 5 to 10 min. Add fresh medium, aspirate and dispense into new flasksSplit ratio: Media change: Reverse transcritase: positiveProduction.