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NFAT Reporter (Luciferase) - THP-1 Cell Line

SKU : BHC18200100

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BPS Bioscience

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Mfr.No.	78320
Description	The NFAT reporter (Luciferase)-THP-1 cell line is designed for monitoring the NFAT (nuclear factor of activated T-cells) signaling pathway in THP-1 cells by measuring luciferase activity. It contains a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter. Upon activation by NFAT activators such as Ionomycin, endogenous NFAT transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. Mycoplasma testing: The cell line has been screened using the PCR-based Venor GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species. Host Species: human Supplied as 2 vials: Each vial contains>1 x 10⁶ cells in 1 ml of Cell Freezing Medium (Biohippo #79796)
Application	Monitor NFAT signaling pathway activity. Screen for compound activity on the NFAT signaling pathway.
Formulation	Thaw Medium 2 (Biohippo, #60184): RPMI1640 medium (Life Technologies, #A10491-01) supplemented with 10% FBS (Life Technologies, #26140-079), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01).Growth Medium 2M (Biohippo, #78181): Thaw Medium 2 (Biohippo, #60184) plus 1 ug/ml of Puromycin (Invivogen, #ant-pr-1).Assay Medium: Thaw Medium 2 (Biohippo, #60184)
Host Cell	THP-1
Synonyms	nuclear factor of activated T cells, T cells transcription factor, NFAT1, NFATP, NFAT2; NFATc, NFATC1, NFATC2
Shipping Temperature	-80°C (dry ice)
Note	Warning: Avoid freeze/thaw cycles.

Storage Stability	Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Reference	1. Clipstone NA, Crabtree GR. Nature. 1992 Jun 25;357(6380):695-7. 2. Lyakh, L., et al. Mol Cell Biol. 1997 May;17(5):2475-84.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200100	78320	2 vials	$1991		Add to Cart

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