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NFAT Luciferase-eGFP Reporter Jurkat Cell Line

SKU : BHC18200167

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BPS Bioscience

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Mfr.No.	78662
Description	Recombinant Jurkat T cells expressing both firefly luciferase and enhanced GFP (eGFP) under the control of NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway can be monitored by examining either firefly luciferase or eGFP expression. Mycoplasma testing: The cell line has been screened to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (Biohippo #79796)
Background	The Nuclear Factor of Activated T Cells (NFAT) family of transcription factors plays an important role in mediating the immune response. T cell activation through the T cell synapse results in calcium influx, which is required to facilitate actin organization and interactions at the synapse. Increased intracellular calcium levels activate the calcium-sensitive phosphatase Calcineurin, which rapidly dephosphorylates the serine-rich region (SRR) and SP-repeats in NFAT proteins. This results in a conformational change that exposes a nuclear localization signal (NLS) promoting NFAT nuclear translocation and inducing gene expression, including various cytokines (IL-2, IL-3, IL-4, and TNF-alpha). Members of the NFAT family have been found in many tissue types, including the heart, skeletal muscle, and brain. These transcription factors are known to be highly involved in the pathological processes of inflammation and cancer. This reporter cell line is designed to monitor T cell activation or inhibition through various checkpoint inhibitors. It can be used as a control or parental cell line to co-express various immune checkpoint inhibitors, such as PD1.
Application	Screen for activators or inhibitors of NFAT signaling pathwayDetermine T cell activation through T Cell Receptor (TCR) or Chimeric Antigen Receptor (CAR)
Host Cell	Jurkat (clone E6-1), human T lymphoblast, suspension
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit Biohippobioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]. Warning: Avoid freeze/thaw cycles.

Storage Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200167	78662	2 vials	$2414.50		Add to Cart

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