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NF-kappaB Reporter (Luc) - HCT116 Cell Line

SKU : BHC18200048

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BPS Bioscience

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Mfr.No.	60623
Description	NF-B luciferase reporter construct is stably integrated into the genome of HCT-116 cells. The firefly luciferase gene is controlled by 4 copies of NF-kappaB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-kappaB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.The NF-kappaB-luciferase/HCT-116 cell line is suitable for monitoring the activity of NF-kappaB signaling in response to stimulants such as the cytokines TNF and IL-1beta, pathogen-associated molecular pattern (PAMP) (i.e. flagellin) or endogenous damage-associated molecular pattern (DAMP) molecules (i.e. NOD1 ligand) (see application references). It is also suitable for establishing cell-based screens for inhibitors that target specific NF-kappaB stimulating molecules. This cell line can be further modified to allow investigation of downstream NF-kappaB activities as a result of targeted genetic mutation(s). Mycoplasma testing: This cell line has been screened using the MycoAlert Mycoplasma Detection Kit (Lonza, Cat. #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, Cat. #LT07-518) was used as a positive control. Host Species: human
Host Cell	HCT116
Synonyms	NF-kappaB cell line, NFkappaB cell line, luciferase reporter, NF-kappaB cell line, NFkB cell line
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc.

Product Format	Aqueous solution containing DMSO
Storage Stability	Store in liquid nitrogen immediately upon receipt.

Reference	1. Samuel T et.al. (2014) Variable NF-B pathway responses in colon cancer cells treated with chemotherapeutic drugs. BMC Cancer 14: 599. 2. Arabi A et.al. (2012) Proteomic screen reveals Fbw7 as a modulator of the NF-B pathway. Nature Communication 3: 976. 3. Clemo NK et.al. (2008) BAG-1 is up-regulated in colorectal tumour progression and promotes colorectal tumour cell survival through increased NF-B activity. Carcinogenesis 29: 849. 4. Tukhvatulin AI et.al. (2011) An In Vitro and In Vivo Study of the ability of NOD1 Ligands to Activate the Transcriptional Factor NF-B. Acta Naturae 3: 77.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200048	60623	2 vials	$1204.50		Add to Cart

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