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NF-kappaB Reporter Cellular Assay Pack (HCT116)

SKU : BHC18200182

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BPS Bioscience

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Mfr.No.	79326
Description	The NF-kappaB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-kappaB) signal transduction pathways. The pack contains the NF-kappaB Reporter (Luc)- HCT-116 Recombinant Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-kappaB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-kappaB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-kappaB inhibitor, evodiamine.Additionally, the pack includes cell culture medium (Thaw Medium 7) that has been optimized for use with HCT-116 cells. Thaw Medium 7 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. Host Species: human
Application	Monitor NF-kappaB-signaling pathway activity Screen inhibitors of NF-kappaB
Host Cell	HCT116
Synonyms	NF-kappaB Cell Line, NFkappaB Cell Line, Luciferase Reporter, NF-kB Cell Line, NFkB Cell Line
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Storage Stability	Immediately upon receipt, store vials in liquid nitrogen.

Reference	1. Samuel T et.al. (2014) Variable NF-kappaB pathway responses in colon cancer cells treated with chemotherapeutic drugs. BMC Cancer 14: 599.2. Arabi A et.al. (2012) Proteomic screen reveals Fbw7 as a modulator of the NF-kappaB pathway. Nature Communication 3: 976.3. Clemo NK et.al. (2008) BAG-1 is up-regulated in colorectal tumour progression and promotes colorectal tumour cell survival through increased NF-kappaB activity. Carcinogenesis 29: 849.4. Tukhvatulin AI et.al. (2011) An In Vitro and In Vivo Study of the ability of NOD1 Ligands to Activate the Transcriptional Factor NF-kappaB. Acta Naturae 3: 77.

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200182	79326	2 vials	$1369.50		Add to Cart

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