Stable recombinant HEK293 cell line expressing tetracycline-inducible human Nav1.8 (Genbank #NP_006505.2) fused to Green Fluorescent Protein (GFP) (Ex. ~395 nm, 475 nm; em ~510 nm) and C-terminal Streptavidin-Binding Peptide (SBP)-tag, and the human sodium channel beta2 subunit (Genbank #NM_004588; SCN2B) with C-terminal FLAG-tag. Nav1.8 is also known as tetrodotoxin-resistant voltage-gated sodium channel type X (SCN10A).
Mycoplasma testing: The cell line is confirmed for absence of Mycoplasma species using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich).
Host Species: human
Background
Nav1.8 is a voltage gated type X, alpha subunit sodium channel which in humans is encoded by the SCN10A gene. It is expressed in nociceptors and has been proposed as a target for the development of new analgesics. Mice deficient in Nav1.8 have deficits in sensing inflammatory pain (initiated by tissue damage/inflammation) and visceral pain (initiated by damage or injury to internal organs) but not neuropathic pain. Native sodium channels are complexes composed of the pore-forming alpha subunit and an auxiliary beta subunit. The sodium channel beta2 subunit, encoded by the SCN2B gene, increases current amplitude and surface expression of sodium ion channels.
Application
Drug compound screening. Functional assays. Efficient antigen for mouse immunization
Host Cell
HEK293
Tag
Nav 1.8: GFP and C-terminal Streptavidin-Binding Peptide (SBP)-tag. beta2 subunit: C-terminal FLAG-tag.
Species
Human
Shipping Temperature
-80°C (dry ice)
Note
License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc.
Product Format
Each vial contains ~1 X 106 cells in 10% DMSO solution
Storage Stability
Immediately upon receipt, store in liquid nitrogen.
Reference
1. Catterall, W.A. et al. Pharmacol. Rev. 57 (4): 397-409 (2005). 2. Muzny, D.M., et al. Nature 440:1194-1198 (2006). 3. Wilson, D.S., et al. Protein Expression and Purification 23 (3): 440-446 (2001).