Recombinant stable clonal CHO cell line constitutively expressing full length human LIGHT protein, also known as TNFSF14, (Genbank #NM_003807). Surface expression is confirmed by flow cytometry.
Mycoplasma testing: This cell line has been screened using the MycoAlert Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
Host Species: hamster
Supplied as Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Background
LIGHT (TNFSF14) is a key component of the communication system that controls the responses of T-Cells. LIGHT activates two cell surface receptors, the Herpesvirus Entry Mediator (HVEM) and the Lymphotoxin-beta Receptor (LTbetaR). The LIGHT-HVEM axis is an important costimulatory pathway for T cells, it has been shown to activate NF-kappaB; LIGHT/HVEM pair are co-stimulatory immune checkpoint molecules for cancer immunotherapy, LIGHT expressing cells have been shown to be more potent than the soluble LIGHT for activating HVEM expressed on T cell surface.
Application
This cell line is useful for LIGHT binding assays, flow cytometry, or for screening for LIGHT antibodies. Also useful for immune checkpoint co-stimulatory assay when co-cultured with HVEM/NF-kappaB-luciferase/Jurkat reporter cell line (Biohippo).
Host Cell
CHO
Synonyms
TNFSF14
Shipping Temperature
-80°C (dry ice)
Note
License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.
Storage Stability
Store in liquid nitrogen immediately upon receipt.
Reference
1. Chen, et.al. (2013) Molecular mechanisms of T cell co-stimulation and co-inhibition. Nat Rev Immunol. 2013 April ; 13(4): 227-242.2. Steinberg, et.al. (2014) The Signaling Networks of the Herpesvirus Entry Mediator (TNFRSF14) in Immune Regulation. Immunol Rev. 2011 November; 244(1): 169-187.3. Rio, et.al. (2014) Therapeutic blockade of LIGHT interaction with HVEM and LTbetaR attenuates in vivo cytotoxic allogeneic responses. Transplantation. 2014 December 15; 98(11): 1165-1174.