The decidual cells play an important role in the maintenance of pregnancy at the maternofetal interface after implantation of the fertilized egg into the endometrium. The Immortalized Rat Decidual Cells (GG-AD) is conditionally immortalized through the temperature-sensitive simian virus (SV40 tsA209) and possesses several endocrinological features, including the expression of progesterone receptor, activin type II, activin betaA and activin betaB. In addition, it also retains the ability to respond to cAMP stimulation and secrete PRL-related hormones, a hallmark of the antimesometrial decidual cells. This cell line is useful for studying the mechanism of decidual cell regression and reorganization.
Cell Type
Immortalized Cells
Disclaimer
1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Level
IL-6, IL-6R, gp130, activin betaA, decidual PRL-related protein (dPRP)
Quantity
1x106 cells / 1.0 ml
Tissue
Reproductive
Morphology
Binucleated|Compact|Polynucleated|Flattened
Species
Rat (R. norvegicus)
Propagation
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow II medium available at abm (TM002). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/streptomycin to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 33.0
Quality Control
1) Northern blot performed to analyze presence of activin betaA , follistatin and PLP-B mRNA; 2) RT-PCR analysis of progesterone receptor, activin betaA, activin betaB, and activin receptor II mRNA was completed with L19 ribosomal protein mRNA as control.
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