Human Peripheral Blood Mononuclear Cells (PBMC) are blood cells with a single round nucleus. These cells include lymphocytes (T, B and NK cells), monocytes and dendritic cells. Mononuclear cells are parts of the immune system which is critical for cell-mediated and humoral immunity. Mobilized peripheral blood is collected after the donor is treated with mobilization agent such as G-CSF. Such treatment mobilizes blood stem cells that are normally reside in the bone marrow to enter the blood stream. The content of CD34+ stem cells in the blood is therefore the most important parameter of graft quality. The optimal CD34+ cell dose is widely accepted to be 5 × 106/kg. Particularly, the more primitive CD34+/CD38-subset has been shown to have high self-renewal and repopulation capability. Stem cell mobilization also affects the properties of other components of the graft such as T lymphocytes and NK cells. AcceGen Human Mobilized PBMCs are isolated from healthy donors that have been mobilized with GCSF collected in acid-citrate-dextrose formula A (ACDA) as an anticoagulant. All donors must be tested negative for HBV, HCV and HIV and are IRB consented.
Categories
Stem Cells
Application
For research use only
Species
Human
Shipping
Dry Ice
Storage
Liquid Nitrogen
Reviews of HighQC Human Mobilized Peripheral Blood Mononuclear Cells