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Firefly Luciferase K562 Cell Line

SKU : BHC18200165

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BPS Bioscience

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Mfr.No.	78621
Description	Recombinant K562 cells constitutively expressing firefly (Photinus pyralis) luciferase under the control of a CMV promoter. Mycoplasma testing: The cell line has been screened to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains 2 x 10⁶ cells in 1 ml of 10% DMSO
Background	The K562 cell line was established from a patient with chronic myelogenous leukemia (CML) in terminal blast crisis. The Firefly Luciferase K562 Cell Line does not express antigens CD19, CD20 or CD22, therefore it makes an excellent negative control in assays that measure specific killing by CAR-T cells targeting B cell antigens CD19, CD20, and CD22. It can also be used as a highly sensitive in vitro target for the natural killer assay. The signal generated by the firefly luciferase reporter is proportional to cell numbers.
Application	1. In vitro and in vivo Bioluminescence Imaging 2. Use as a negative control in CAR-T or NK co-culture killing assays
Formulation	For best results, it is highly recommended to use these validated and optimized media from Biohippo. Other preparations or formulations of media may result in suboptimal performance. Thaw Medium 2 (Biohippo #60184): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.Growth Medium 2D (Biohippo #79639): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 200 ug/ml of Hygromycin B.
Host Cell	K562, a human myelogenous leukemia cell line derived from a patient with CML; suspension.
Species	Photinus pyralis
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit Biohippobioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]. Warning: Avoid freeze/thaw cycles.

Storage Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200165	78621	2 vials	$2090		Add to Cart

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