Spleen cells were fused with FOX-NY myeloma cells; The antibody reacts with several phosphorylated proteins on Western blots and in immunoprecipitation reactions; Thymidine and aminopterin are added to the culture medium to stabilize the presence of the immunoglobulin heavy chain locusTumorigenecity: Isoenzyme: IgG3; Kappa light chainHistopathology: hybridoma; Mouse(myeloma)Subculture: Split ratio: Media change: Reverse transcritase: Productionimmunoglobulin; Monoclonal antibody; Against phosphotyrosine.