Recombinant stable NK-92 cell line constitutively expressing enhanced Green Fluorescent Protein (eGFP). The cell line was generated using the eGFP Lentivirus ( Biohippo #79979). Expression is driven by a CMV promoter.
Mycoplasma testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Host Species: human
Supplied as Each vial contains 2 x 106 cells in 1 ml of cell freezing medium (Biohippo, #79796)
Background
T cells expressing Chimeric Antigen Receptors (CARs) have shown promising results in targeting antigen-specific tumor cells. However, CAR-T cell therapy comes with specific risks such as Graft versus Host Disease or cytokine storm. Natural Killer (NK) cells are also cytotoxic to cancer cells and can make a good alternative to CAR-T with lesser risk or side effects. NK-92 cells are a human Interleukin-2 (IL-2)-dependent NK cell line derived from a patient with non-Hodgkin's lymphoma. NK-92 cells can be used to generate Chimeric Antigen Receptor-modified Natural Killer cells (CAR-NK).
Application
Use as a control for NK cell killing assaysUse as a platform to generate Chimeric Antigen Receptor-modified Natural Killer Cells (CAR-NK)
Host Cell
THP-1, monocyte, suspension
Shipping Temperature
-80°C (dry ice)
Note
License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit Biohippobioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]. Warning: Avoid freeze/thaw cycles.
Storage Stability
Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.