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CSL Reporter - HEK293 Cell line

SKU : BHC18200212

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BPS Bioscience

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Mfr.No.	79754
Description	The Notch CSL Reporter - HEK293 cell line contains the firefly luciferase gene under the control of Notch-response elements (CSL responsive elements) stably integrated into HEK293 cells. Transfection of this cell line with a Notch expression vector and activation of the Notch pathway leads to expression of luciferase reporter. This cell line is validated with a Notch1DelE expression vector, which is constitutively processed by gamma-secretase, leading to the release of NICD and the expression of luciferase. Host Species: human Supplied as Each vial contains ~1.5 X 106 cells in 1 ml of FBS with 10% DMSO.
Background	The Notch signaling pathway controls cell fate decisions in vertebrate and invertebrate tissues. Notch signaling is triggered through the binding of a transmembrane ligand to Notch transmembrane receptor (NOTCH1/ NOTCH2/NOTCH3/NOTCH4) on a neighboring cell. This results in proteolytic cleavage of the Notch receptor, releasing the constitutively active intracellular domain of NOTCH (NICD). NICD translocates to the nucleus and associates with transcription factors CSL (CBF1/RBPJkappa/Suppressor of Hairless/Lag-1) and coactivator Mastermind to turn on transcription of Notch-responsive genes.
Application	Monitor Notch signaling pathway activity. Screen for inhibitors of the Notch signaling pathway Test synthetic Notch receptors (synNotch) or other genetic constructs that conditionally activate the CSL promoter
Host Cell	HEK293
Synonyms	Notch reporter, PKA reporter, cAMP reporter, luciferase reporter , CSL reporter, CBF1 reporter, RBP-J reporter, Cell based assay kit, cell-based assay, reporter assay kit
Shipping Temperature	-80°C (dry ice)
Note	License DisclosurePurchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc.

Storage Stability	Immediately upon receipt, store cells in liquid nitrogen.

Reference	Lu, F.M., et al. (1996) Constitutively active human Notch1 binds to the transcription factor CBF1 and stimulates transcription through a promoter containing a CBF1-responsive element. Proc. Natl. Acad. Sci. USA 93(11): 5663-5667. Kanungo, J., et al (2008) The Notch signaling inhibitor DAPT down-regulates cdk5 activity and modulates the distribution of neuronal cytoskeletal proteins. J. Neurochem. 106: 2236-2248.

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200212	79754	2 vials	$2502.50		Add to Cart

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