The Cells are free of bovine viral diarrhea(BVD) virus.Tumorigenecity: Isoenzyme: Histopathology: normalSubculture: Remove medium, add fresh 0.25 % Trypsin 0.02 % EDTA solution for 1 to 2 minutes, remove trypsin and allow culture to stand at room temperature ( or at 37'C)until cells detach. Add fresh medium aspirate and dispense into new flasks.Split ratio: Media change: Reverse transcritase: negativeProduction.