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CGRPR/CRE Luciferase Reporter HEK293 Cell Line

SKU : BHC18200104

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Mfr.No.	78325
Description	Recombinant HEK293 cell line stably expressing full-length human Calcitonin receptor-like receptor (CALCRL/CRLR/CLR; accession number: NM_005795) and containing a firefly luciferase gene under the control of multimerized cAMP response element (CRE). This cell line can be used to measure the EC50 and IC50 of CALCRL agonists and antagonists using the luciferase reporter activity. Mycoplasma testing: The cell line has been screened to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains 2 x 10⁶ cells in 1 ml of 10% DMSO
Background	Calcitonin gene-related peptide (CGRP) receptor is a G-protein coupled receptor with three components: calcitonin receptor-like receptor (CALCRL), receptor activity-modifying protein (RAMP1) and CGRP-receptor component protein (CRCP). RAMP1 is required for both the trafficking of the calcitonin-like receptor to the plasma membrane and the coupling of the receptor with CGRP agonist. CRCP is involved in the association between the CGRP receptor and the G protein. Upon ligand binding, the complex interacts with the G protein, the Galphas subunit dissociates from the complex and activates adenylyl cyclase, which produces cAMP. Elevation of the intracellular concentration of cAMP stimulates cAMP-dependent signaling pathways, ultimately resulting in transcription factor cAMP response element binding protein (CREB) to bind to the CRE promoter and induce gene expression.
Application	Screen for activators or inhibitors of CALCRL for immunotherapy research and drug discovery. Characterize CALCRL antibodies, antagonists and ligands for binding assay.
Formulation	For best results, it is highly recommended to use these validated and optimized media from Biohippo. Other preparations or formulations of media may result in suboptimal performance. Media Required for Cell Culture Thaw Medium 1 (Biohippo #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin.Growth Medium 1G (Biohippo #79544): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 400 ug/ml of Geneticin and 50 ug/ml of Hygromycin B.
Host Cell	HEK293
Species	Human
Synonyms	Calcitonin gene-related peptide type 1 receptor, CGRP type 1 receptor, Calcitonin receptor-like receptor, CRLR, CGRPR, LMPHM8, CALCRL/CRE Luciferase Reporter HEK293 Cell Line
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit Biohippobioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]. Warning: Avoid freeze/thaw cycles.

Storage Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200104	78325	2 vials	$11374		Add to Cart

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