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CFTR - HEK 293 Cell Line

SKU : BHC18200015

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BPS Bioscience

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Mfr.No.	60506
Description	The CFTR HEK293 Cell Line expresses full length, wild-type human cystic fibrosis transmembrane conductance regulator (CFTR) protein (Genbank #P13569, NP_000483.3), with a C-terminal Streptavidin-Binding Peptide (SBP) tag.Expression must be induced>=24 hours prior to an experiment using 1 ug/ml Doxycycline and 3 mM Na-butyrate. The inducible expression of CFTR was confirmed by Western blotting and flow cytometry. Host Species: human Supplied as Each vial contains ~2 X 10^6 cells in 1 ml of Sigma Freezing Medium (Cat. # C-6164).
Background	Cystic fibrosis transmembrane conductance regulator (CFTR) is a protein that in humans is encoded by the CFTR gene. CFTR is an ABC transporter-class ion channel that transports chloride and thiocyanate ions across epithelial cell membranes. Mutations of the CFTR gene affect functioning of the chloride ion channels in these cell membranes, leading to cystic fibrosis. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes.
Application	Compound or antibody screening Functional assays for CFTR Antigen preparation for mouse immunization
Biological Activity	The expression of CFTR is confirmed by Western blotting and flow cytometry (FACS).
Host Cell	HEK293
Species	Human
Synonyms	CFTR, cystic fibrosis transmembrane conductance regulator
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Storage Stability	Store in liquid nitrogen immediately upon receipt. Cells are stable after a minimum of seven continuous passages. Upon first use, amplify the cells in culture and make several frozen aliquots for future use.

Reference	1. Gadsby, D.C., et al. Nature 440 (7083): 477483 (2006).2. Hillier, L.W., et al. Nature 424:157-164 (2003).3. McCann, C. M., et al. BioTechniques 38 (6):945952 (2005).

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200015	60506	2 vials	$8250		Add to Cart

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