Recombinant CHO cell line stably expressing full length human CD43 gene (sialophorin, gpL115, leukosialin, SPN; ref. seq. NM_003123) under the control of the CMV promoter.
Mycoplasma testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Host Species: hamster
Supplied as Each vial contains ~2 x 106 cells in 1 ml of 10% DMSO
Background
CD43 is a heavily glycosylated type I transmembrane protein expressed on the surface of most hemopoietic cells. It is abundantly expressed on the surface of T cells and functions as a co-stimulatory molecule by transducing activation signals through its cytoplasmic domain. Ultimately, it is thought that CD43 contributes to downstream T cell gene regulation and modulates cell function.
Application
Screen for antibodies binding to CD43 or examine CD43 function in a cellular context
Formulation
For best results, it is highly recommended to use these validated and optimized media from Biohippo. Other preparations or formulations of media may result in suboptimal performance. Media Required for Cell Culture Thaw Medium 3 (Biohippo #60186): F-12K medium supplemented with 10% FBS, 1% Penicillin/StreptomycinGrowth Medium 3D (Biohippo #79539): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 mg/ml Geneticin
License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit Biohippobioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]. Warning: Avoid freeze/thaw cycles.
Storage Stability
Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.