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CD4+ T cells, Negatively Selected (Human)

SKU : BHC18200210

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BPS Bioscience

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Mfr.No.	79752
Description	Cryopreserved vial (10 x 10^6 cells) of CD4+ T cells that were negatively selected from freshly isolated primary human peripheral blood mononuclear cells (PBMCs). The PBMCs came from a healthy donor, and were isolated from whole blood or leukapheresis samples using a Ficoll gradient. Magnetic antibodies to monocytes, granulocytes, CD8+ T cells, gamma/delta T cells and other immune subsets present in PBMCs were then used to purify untouched CD4+ T cells via immunomagnetic separation. Before and after CD4+ T cell isolation, the cells were stained to evaluate purity and viability by flow cytometry. Cells were cryopreserved in CryoStor CS10 cryopreservation medium (Stemcell, #07930) at a controlled rate.Source Normal human PBMC from Leukapheresis Sample Host Species: human
Synonyms	T-Cell Surface Glycoprotein CD4, T-Cell Surface Antigen T4/Leu-3, CD4 Molecule
Shipping Temperature	-80°C (dry ice)
Note	Characterization Criteria Cell count, viability (trypan blue exclusion and FACS with impermeable DNA binding dye), and surface expression of CD3 and CD4

Storage Stability	Store cells at -135°C or colder. Thawed cells should be used immediately for downstream applications. Because these are primary cells, we do not recommend maintaining these cells in culture for long periods of time.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200210	79752	10 million cells	$434.50		Add to Cart

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