Recombinant CHO-K1 cells constitutively expressing human B7-H7 (also known as HHLA2: HERV-H LTR-associating protein 2, GenBank accession #NM_007072) and an engineered T cell receptor (TCR) activator. B7-H7 mediates an immune-stimulatory signal via TMIGD2 (Transmembrane and immunoglobulin domain containing 2) in naA¯ve T cells while it delivers an immune-inhibitory signal through KIR3DL3 in activated T cells and Natural Killer (NK) cells.
Mycoplasma testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Host Species: hamster
Supplied as Each vial contains 2 x 106 cells in 1 ml of 10% DMSO
Application
Characterize the biological activity of KIR3DL3 or TMIGD2 interactions with B7-H7 Screen for B7-H7 antibodies
Formulation
For best results, it is highly recommended to use these validated and optimized media from Biohippo. Other preparations or formulations of media may result in suboptimal performance. Media Required for Cell Culture Thaw Medium 3 (Biohippo #60186): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.Growth Medium 3A (Biohippo #60188): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 mg/ml of Geneticin and 500 ug/ml of Hygromycin.Media Required for Cellular Assay Thaw Medium 2 (Biohippo #60184): RPMI1650 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.
Host Cell
CHO
Species
Human
Synonyms
HERV-H LTR-associating protein 2, Human endogenous retrovirus-H long terminal repeat-associating protein 2, B7y, B7H7, B7-H5, B7-H7
Shipping Temperature
-80°C (dry ice)
Note
License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit Biohippobioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]. Warning: Avoid freeze/thaw cycles.
Storage Stability
Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Reference
1. Bahatt RS, et. al., Cancer Immunol Res. 2021 Feb;9(2):156-169.