Amylin 3 Receptor cell line

Figure 1. Response of ACTOne AMY3 cell line & parental cell line to amylin and calcitonin. ACTOne AMY3 cells and parental cells (AG-10200-258) were plated overnight in 20 ml culture medium on a BD Biocoat 384 well plate. The next day, cells were dye-loaded with 20 ml/well of 1X Dye-loading solution (ACTOne Membrane Potential Assay Kit). After 2 hours of incubation at room temperature, two readings were obtained prior to and 30 min after the addition of rat amylin or hCT. Ratios of the two readings (F/F0) are plotted in the figure. A. Dose response curve of rat amylin and human calcitonin in ACTOne AMY3 cell line. In the presence of PDE inhibitor Ro20-1724, EC50 = 7.2 pM with rAmy and EC50 = 934 pM with hCT. B. Dose response curve of rat amylin and human calcitonin in Parental cells. In the presence of PDE inhibitor Ro20-1724, EC50 = 230 pM with rAmy and EC50 = 28.7 pM with hCT

No Description Available.