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Home / Primary Antibodies

CD20 Antibody (V2048CF488)

SKU : BHA17123358

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NSJ Bioreagents

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Mfr.No.	V2048CF488-100T
Clonality	Monoclonal (mouse origin)
Description	Recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
Formulation	500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Host	Mouse
Immunogen Region	The lymphoblastoid cell line Daudi was used as the immunogen for this CD20 antibody.
Isotype	Mouse IgG2a, kappa
Species Reactivity	Human
Note	Optimal dilution of the CD20 antibody should be determined by the researcher.

Uniprot	P11836

Format	CF488 Conjugate
Purity	Protein G affinity chromatography
Storage	Store the CD20 antibody at 2-8oC, protected from light.

Applications	FACS
Description	Flow cytometry: 5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17123358	V2048CF488-100T	100 Tests	$499		Add to Cart

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