Human Caspase 9 (CASP9), GenBank Accession No. NM_001229, a.a. 1 - 416 (end), with C-terminal His-tag, MW=35 &10 kDa, expressed in an E. coli cell expression system. Procaspase-9 is autocleaved between the prodomain and large protease subunit (p35). The cleavage product contains both the large (p35) and small (p10 (His-tag)) protease subunits.
Specific activity: >= 0.11 pmol/min/ug
Immunogen Region
1 - 416 (end)
Tag
C-terminal His-tag
Formulation
50 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT,80 mM imidazole.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
50 mM HEPES, pH 7.2,50 mM NaCl, 10 mM EDTA, 0.1% Chaps,5% glycerol, 10 mM DTT, and 10 uMCaspase 9 substrate. Incubate for 30minutes at room temperature. Fluorescenceintensity is measured at exc380/em505 nm.
Avoid freeze/thaw cycles.
GenBank
NM_001229
Mw(kda)
see Description
Synonyms
Caspase-9, CASP9, Caspase 9, Mch6, ICE-LAP6
Uniprot
P55211
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Guerrero AD., et al., J Clin Cell Immunol.9;3(3) (2012) 2. Luo W., et al., Int J Biol Macromol. S0141-8130(13)00222-5. (2013)