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AAV Products and Services

At Biohippo, we specialize in providing both pre-made and customized adeno-associated virus (AAV) products, catering to a diverse range of research. Our extensive catalog features over 1,000 pre-made AAV products, each standardized with a high titer of 2E+12 vg/ml, ensuring robust performance for various applications.

For those seeking tailored solutions, our customized AAV services stand out in the field. We offer a wide selection of over 50 promoter options and more than 22 serotypes, allowing researchers to create AAV preparations that perfectly match their specific experimental requirements.

Table 1. The general information about AAV products

Application Titer Range Volume Total GC Turnaround
Cell culture 2~5 x 1012 vg/ml 200 μl ≥4.00E+11 3~4 Weeks
2~5 x 1012 vg/ml 500 μl ≥1.00E+12 3~4 Weeks
2~5 x 1012 vg/ml 1 ml ≥1.00E+12 3~4 Weeks
2~5 x 1012 vg/ml 5 ml ≥1.00E+13 5~6 Weeks
2~5 x 1012 vg/ml 10 ml ≥2.00E+13 6~7 Weeks
2~5 x 1012 vg/ml 20 ml ≥4.00E+13 6~7 Weeks
5~9 x 1012 vg/ml 1 ml ≥5.00E+12 3~4 Weeks
Cell culture & in vivo 1×1013 vg/ml 100 μl ≥1.00E+12 2~3 Weeks
1×1013 vg/ml 200 ul ≥2.00E+12 2~3 Weeks
1×1013 vg/ml 500 ul ≥5.00E+12 3~4 Weeks
1×1013 vg/ml 1 ml ≥1.00E+13 3~4 Weeks
1×1013 vg/ml 5 ml ≥5.00E+13 4~5 Weeks
1×1013 vg/ml 10 ml ≥1.00E+14 4~5 Weeks

Table 2. An overview of AAV serotypes

Regular Serotype
AAV1
AAV2
AAV3
AAV4
AAV5
AAV6
AAV6.2
AAV7
AAV8
AAV9
AAV rh10
AAV DJ
AAV DJ/8
PHP.S
AAV2-retro
AAV2-QuadYF
AAV2.7m8
New Serotypes Tissue
AAV/11 AAV11 permits efficient retrograde targeting of projection neurons
AAV/B10 CNS, suitable for the central nervous system, can cross the blood-brain barrier
AAV/B22 CNS, suitable for the central nervous system, can cross the blood-brain barrier
AAV/Mac Efficiently transfects the central nervous system of non-human primates and can cross the blood-brain barrier (intravenous injection)
AAV/BR1 Targeting brain vascular endothelial cells
AAV/BI30 Targeting brain vascular endothelial cells

Pre-made AAVs

Our pre-made AAV product is a convenient and efficient solution for researchers. These products are available in a ready-to-use format, minimizing preparation time and accelerating the pace of research. The actual titer will be reported with each shipment.

Table 3. AAV Products categorized by function

Chemogenetics AAV
Receptor Promoter Tag Activity Serotype
Activating Synapsin Fusion tags Cre-dependent AAV1
hM3D(Gq) DREADD CaMKIIa dTomato Flp-dependent AAV2
rM3D(Gs) DREADD CD68 EGFP Constitutive (non-cre dependent) AAV5
Inhibitory Dlx HA AAV8
hM4D(Gi) DREADD E2 regulatory element mCherry AAV9
KOR DREADD GFAP mCitrine Retrograde AAV
PSAM4 GlyR Non-fusion tags PHPeB
Biosensor AAV
Calcium Sensors Promoter Tag Activity Serotype
GCaMP5G CAG,CAMKIIa,CMV,CRH dTomato Cre-dependent AAV1
GCaMp6s EF1α,GfaABC1D,GFAP,hSyn Flag-HA Flp-dependent AAV5
GCaMp6f mTH,nEF1α,OT,OXTR Cre and Flp-dependent AAV8
GCaMp6m PV Cre, Flp, and VCre-dependent AAV9
jGCaMp7 thy1 Constitutive (non-cre dependent) Retrograde AAV
CaMPARI TRE3g PHPeB
jRGECO1, jRCaMP1 TRPV1
Neurotransmitter sensor
Neurotransmitter sensors GABA Sensor ATP Sensors Other Sensors Promoter
Dopamine Sensors iGABASnFR ATP1.0 CCK2.0 CAG
dLight1.1 Acetylcholine Sensors ATP1.0_mut AVP1.5 EF1α
DA1m ACh2.0 ATP1.1h eCB1.0 GfaABC1D
DA1h ACh4.3 ATP1.1m MT1.5 hSyn
DA1h mut ACh4.3_mut Serotonin Sensors SST1.5
DA4.4 Norepinephrine (NE) Sensors 5HT2.1(mutant) VIP1.0
Glutamate Sensor NE1m 5HT2.1
iGluSnFR(A184S) NE1h 5HT2B
iGluSnFR(A184S) NEmut 5HT6
iGluSnFR(A184S) NE3.1
Adenosine Sensors
Ado1.0
Ado1.0mut
Optogenetics AAV
Opsin Promoter Fluorophore Activity Serotype
Excitatory TBG GFP Cre dependent AAV1
Wild-type ChR2 CAG EGFP Flp dependent AAV2
ChR2/H134R CaMKIIa EYFP Cre and Flp dependent AAV5
soCoChR CMV mCherry Constitutive AAV8
ChR2/other mutants CS-CRM4-aMHC tdTomato AAV9
Chronos, Chrimson, ChRmine, ChroME, ChETA, C1V1 (t/t) D1 YFP AAVrg
Inhibitory D2 citrine PHPeB
Arch and variants EF1α mOrange
eNpHR and variants GFAP
Jaws hSyn
stGtACR Ki67
iC++ L7
OPN3 mDLx
PPO nEF1α
Bidirectional PTH
BiPOLES RAM
TRE3g
TRPV1
VGAT1
Recombinase
Recombinases Promoter Tag
Cre CMV EF1α Mash1 3XFlag
CAG E-SARE mCD68 dsRed
CaMKIIa ETV1 mDlX EGFP
CAR foxb1 Mecp2 EYFP
CB1 fPV mOXT Flag
CBh fSST mTH mCherry
cfos GAD67 Oxytocin
ChAT GFAP PTH
CRH GLP PV
CS-CRM4-αMHC Grm6 RK
CX3CR1 GRP TBG
D1 hCD68 TH
D1(SP) HDC Tmem119
D2 hSyn TPH2
D2(ENK) Hypocretin TRE3G
DBH KI-67 TRPV1
Dlx5/6 L7 VGAT1
VGAT2
CreERT2 cfos Flag
E-SARE
FLP CMV hSyn CAG EGFP
EF1α TRE CaMKIIa mCherry
VGLUT1

Table 1. Viral vectors for neuronal Tracing research

Type Virus name Classification Genomic type
Non- transsynaptic Adeno-associated virus, AAV Parvoviridae Single-stranded DNA
Canine adenovirus, CAV Adenovirus Double-stranded DNA
Semliki Forest virus, SFV Togaviridae + Single-stranded RNA
Rabies virus (Glycoprotein G-deleted), RV-∆G Rhabdoviridae -Single-stranded RNA
Herpes simplex virus amplicon, HSV amplicon Herpesviridae Double-stranded DNA
Trans-synaptic Trans multisynaptic Anterograde, multisynaptic Herpes simplex virus, HSV H129 Herpesviridae Double-stranded DNA
Vesicular stomatitis virus, VSV Rhabdoviridae -Single-stranded RNA
Retrograde, multisynaptic Pseudorabies virus, PRV Herpesviridae Double-stranded RNA
Rabies virus, RV WT Rhabdoviridae -Single-stranded RNA
Trans-monosynaptic Anterograde, monosynaptic Herpes simplex virus (TK-deleted), HSV-∆TK Herpesviridae Double-stranded DNA
Adeno-associated virus, serotype 1, AAV1 Parvoviridae Single-stranded DNA
Retrograde, monosynaptic Rabies virus, RV-∆G-EnvA Rhabdoviridae -Single-stranded RNA
Pseudorabies virus (TK-deleted), PRV-∆TK Herpesviridae Double-stranded RNA​

Customized Services

In order to meet the needs of our customers, we offer a wide variety of options for AAVs. Researchers can select from an array of promoters and serotypes to create AAV vectors that are precisely tailored to their study's objectives. Each customized service undergoes a strict quality control process to ensure that the viral particles you receive are of the highest quality.

How to design AAV-GOI/shRNA vector

Step 1   Select a promoter

Step 2   Confirm the ORF/ncRNAs

Step 3   Fusion or non-fusion expression

Step 4   Add reporter/Affinity tag, and signal peptide, etc.

Step 5   Add WPRE and terminator

AAV Helper-Free System

Customized Service Production Process

Request and Production Guide

  • Submit your needs to us by clicking the Send Request button or email us at [email protected] and our scientific and production team will design your vector and provide you with a price quote.
  • Or, for any customers who decide to make use of viruses as nucleotide sequence delivery tools, our scientists will work with you step by step from the design of a study to viral manufacturing to facilitate your research.

Resources

Three Research Tools for Neurodegenerative Diseases.

An Effective New Adeno-Associated Virus (AAV) Serotype - AAV13.

Detection of titer and purity of recombinant AAV.

How to read rAAV Vector Map?

Free Sample Request

Sample Type: Pre-designed AAV products
Sample size: 5ul
The customer is responsible for the cost of shipping.

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