The KD values of D13-9001 in Escherichia coli and Pseudomonas aeruginosa were 1.15uM and 3.57uM, respectively. D13-9001 has antibiotic activity. It is an effective AcrB (AcrAB-TolC efflux pump subunit) and MexB (MexAB-OprM efflux pump subunit) inhibitor.