D-Luciferin, Sodium Salt D

Mfr.No.

40901ES03, 40901ES01, 40901ES02

Description

D-luciferin is a common substrate for Luciferase and is widely used throughout biotechnology, especially in vivo imaging technology. The mechanism of action is that luciferin (the substrate) is oxidized to emit light in response to ATP and luciferase (see figure below). It's produced when there's too much luciferin The light quantum number was positively correlated with the concentration of luciferase. Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice In vivo, fluorescein is then injected and changes in light intensity are detected using bioluminescence imaging (BLI) to monitor disease progression or drug efficacy in real time and so on. ATP's influence on the reaction system can also be used to indicate energy or vital signs according to changes in bioluminescence intensity.

D-luciferin is also commonly used in in vitro studies, including luciferase and ATP levels analysis; Reporter gene analysis; High-throughput sequencing and various contamination tests. There are currently three product forms: D-luciferin (free acid), D-luciferin salts (sodium and potassium salts). The main difference lies in solubility: the former is water-soluble and the solubility of buffer system is weak, except soluble in weak bases such as low concentration NaOH and KOH solutions, soluble in methanol and DMSO; The latter is easily soluble in water or buffer, easy to use, solvent non-toxic, especially suitable for in vivo experiments. As a solution, there is no substantial difference between the three products in the vast majority of applications

Applications

In the tumorigenesis experiment in nude mice, the tumor growth was observed without invasion in real time, without tumor stripping measurement.
To test the effect of the administration on tumor growth or metastasis, the fluorescein substrate can be completely eliminated within 3 hours, without interference to the drug experiment.
The localization and distribution of foreign cells in animals were detected.
The target gene or promoter of the target gene is fused to the luciferase gene to detect changes in gene expression during drug treatment or disease progression.
Monitoring stem cell transplantation, survival and proliferation; Trace the distribution and migration of stem cells in vivo.

Alternative Names

(S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt; D-Luciferin firefly, sodium salt monohydrate;

Appearance

Light yellow powder

Features

No radiation, almost harmless to living organisms.
Bioluminescence, no excitation light source.
So sensitive, you can detect it in a few hundred cells.
Good penetration, 3-4cm tissue depth can still be detected.
High signal-to-noise ratio, strong fluorescence signal and good anti-interference.

Kit Components

Components No.	Name	40901ES01	40901ES02	40901ES03	40901ES08	40901ES10
40901	D-Luciferin, Sodium Salt	100mg	500 mg	1g	5g	10g

Molecular Formula

NaC11H7N2O3S2·H2O

Cas Number

103404-75-7

Molecular Weight

320.32 g/mol

Solubility

Solube in water(100 mg/mL)

Purity	≥95%
Storage	The product should be stored at -15℃ ~ -25℃ for 1 year.

Product Manual	40901_MSDS_HB220415_EN_1652931562564.PDF 40901_MSDS_HB220422_EN_1652931562524.PDF 40901_MSDS_HB220422_EN_1652931562832.PDF Manual

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Combination SKU	Supplier No.	Size	Your price
BHB20800006-1	40901ES03	1 g	$1525	Add to Cart
BHB20800006-2	40901ES01	100 mg	$195	Add to Cart
BHB20800006-3	40901ES02	500 mg	$845	Add to Cart

D-Luciferin, Sodium Salt D

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