Abbkine AbFluor 594-Phalloidin selectively bound to F-actins.
Alternative Names
AbFluor 594-Phalloidin,BMD00084
Formulation
Lyophilized powder
Features
Optimized staining protocol for labeling, identifying and quantitating F-actin in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. <br/> Proprietary AbFluor 594-Phalloidin (Red Fluorescence) (Ex/Em = 590/617 nm)-high-affinity probe for F-actins and much higher photostability than the other fluorescein-phalloidin conjugates.
Notes
AbFluor 594-Phalloidin selectively bound to F-actins, it is much higher photostability than the fluorescein-phalloidin conjugates. Phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, which can stain F-actins in cell cultures or cell-free experiments at nanomolar levels. <br/><br/>Phalloidin is toxic, it should be handled with care.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Phalloidin belongs to a class of toxins called phallotoxins, which are isolated from the death cap mushroom (Amanita phalloides). It is a bicyclic peptide that binds to F-actin specifically. Therefore, the distribution of F-actin can be very conveniently studied by using a fluorescent dye-labeled phalloidin. Inside the phalloidin, there is an unusual thioether bridge between cysteine and tryptophan, which can form an inner ring structure. When the pH is raised, the thioether is cleaved and the phalloidin loses its affinity for actin.
Tag
F-actin
Storage
Stable for at least 12 months if store at -20°C. Protect this product from light.